InhaltsangabePreface Foreword Abbreviations Glossary of terms INTRODUCTION History Critical points Hypothesis driven approach vs. High Throughput Complementarities of workflows Concept of experimental planning Success stories, highlights of useful references PART I: PROTEOMICS TECHNOLOGY Electrophoresis Liquid Chromatography Mass spectrometry Bioinformatics tools / data mining Methods under development PART II. PRACTICAL MANUAL OF PROTEOME ANALYSIS Strategy where to start Gelbased workflow LCbased workflow Latest trends Validation with Western blotting Statistic evaluation PART III: TROUBLE SHOOTING Gelbased workflow LC based workflow References Index

Reiner Westermeier gained his doctorate in 1981 at the Technical University of Munich, Germany. There he remained engaged in the development of new electrophoresis systems and applications until 1983, a period that also involved several international collaborations and lecturing tours. From 1984 to 1987 Reiner Westermeier was employed as an electrophoresis products and applications specialist at LKB Instrument GmbH thereafter until 1990 at Pharmacia Biotech. He founded ETC Elektrophorese-Technik in 1991 for the development of new electrophoresis methods, media, and equipment. From January 1996 to July 1997 he was manager of scientific support Europe at Pharmacia Biotech in Freiburg, Germany. He has been at Amersham Pharmacia Biotech, later GE Healthcare since July 1997, and its Senior Scientist for Proteomics since January 2002. Reiner Westermeier is the author of several publications and two books, both published by WILEY-VCH. Tom Naven and Hans-Rudolf Höpker are also working for GE Healthcare as proteomics scientists.

Preface Foreword Abbreviations Glossary of terms INTRODUCTION History Critical points Hypothesis driven approach vs. High Throughput Complementarities of workflows Concept of experimental planning Success stories, highlights of useful references PART I: PROTEOMICS TECHNOLOGY Electrophoresis Liquid Chromatography Mass spectrometry Bioinformatics tools / data mining Methods under development PART II. PRACTICAL MANUAL OF PROTEOME ANALYSIS Strategy where to start Gel-based workflow LC-based workflow Latest trends Validation with Western blotting Statistic evaluation PART III: TROUBLE SHOOTING Gel-based workflow LC based workflow References Index